In vivo control of perilla rust disease by oak pyroligneous liquor

Leaves of Perilla frutescens, commonly known as perilla are widely used in Korea, due to their medicinal properties. The rust disease of perilla interrupts its wide use every year. The present study was carried out to evaluate the control of perilla rust disease by using oak pyroligneous liquor having various biological properties. The rust disease infected perilla leaves were applied with 100 μl of oak pyroligneous liquor at different concentrations such as 6.25, 12.5, 25 and 50 μg/ml. Then the leaf samples were observed for the abortion of active rust pustules (yellow color) after 48 hours of incubation at 26 ̊C. Further, inhibition of rust pustules development by oak pyroligneous liquor was evaluated on the leaves of artificially induced perilla rust disease. The best abortion of rust pustules by oak pyroligneous liquor was noticed at the concentration of 50 μg/ml, where 74.4% of the pustules were aborted. Discoloration of rust pustules was also observed in the treated leaves from yellow to white when compared with the untreated control. In addition, oak pyroligneous liquor completely inhibited the development of rust pustules in artificially induced leaves even at low concentration (6.25 μg/ml). The results clearly indicated that oak pyroligneous liquor is a good candidate for the management of Perilla rust disease.